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Algorithms for correcting uneven lighting in images of parallel DNA sequencing systems
V.V. Manoilov1, A.G. Borodinov1, A.I. Petrov1, I.V. Zarutsky1, A.S. Saraev1, V.E. Kurochkin1

1IAI RAS - Institute for Analytical Instrumentation of the Russian Academy of Sciences, 198095, Russia, St. Petersburg, Ivan Chernykh St. 31-33 lit. A

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DOI: 10.18287/COJ1691

Article ID: 1691

Language: English

Abstract:
The uneven illumination that occurs in the images obtained during experiments on the analysis of nucleic acids in the flow cell of the parallel sequencing system "Nanofor SPS" leads to the appearance of bias, which distorts the intensity and makes it difficult to quantify the fluorescent signals. Uneven lighting can cause the intensity of an object in one area of the visual field to be measured differently from the intensity of an object with an equal concentration of fluorophore in another area of the visual field. The paper describes methods for correcting the additive and multiplicative components of factors that distort the measured images. The results of research on the evaluation of parameters of correction algorithms are presented. The number of fragments of nucleic acids is used as a criterion for comparing various parameters of algorithms for correcting uneven lighting, which is selected by a program that assembles the whole genome from its individual parts. The more such fragments there are, the fewer errors there are in the results of genome construction.

Keywords:
background correction, sequencing, image processing, information optical technologies.

Acknowledgements:
This work was supported by the Ministry of Science and Higher Education within the State assignment № 075-00444-25-00 (by 26.12.2024).

Citation:
Manoilov VV, Borodinov AG, Petrov AI, Zarutsky IV, Saraev AS, Kurochkin VE. Algorithms for correcting uneven lighting in images of parallel DNA sequencing systems. Computer Optics 2026; 50(1): 1691. DOI: 10.18287/COJ1691.

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