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Color models of interference images of thin stratified objects in optical microscopy

A.A. Dyachenko1,2, V.P. Ryabukho1,2

1 Saratov State University, Saratov, Russia,
2 Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russia

 PDF, 1110 kB

DOI: 10.18287/2412-6179-2019-43-6-956-967

Pages: 956-967.

Full text of article: Russian language.

Abstract:
Algorithms for the analysis of polychromatic interference patterns in images of thin stratified objects in optical microscopy are considered. The algorithms allow one to measure the thin-film optical thickness. A measurement method based on the comparison of colors of the interference image under study and  a numerically simulated image is discussed. We discuss a mathematical model for the calculation and numerical simulation of interference patterns and algorithms for interference pattern processing. Color comparison in an RGB color model is described and limitations of such a method are shown. The feasibility of using a Lab color model is shown and algorithms of interference color comparison in this model are presented. Results of application of the presented algorithms to measuring the optical thickness of red blood cells in a blood smear are discussed. The estimation of the error and robustness of the proposed algorithms is conducted.

Keywords:
interference colors, thin films, optical microscopy, interference microscopy, colorimetry, color comparison, color model.

Citation:
Dyachenko AA, Ryabukho VP. Color models of interference images of thin stratified objects in optical microscopy. Computer Optics 2019; 43(6): 956-967. DOI: 10.18287/2412-6179-2019-43-6-956-967.

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